![]() Since the distinctness of Thymus species from another is always challenging to identify, several characters might need to be considered. The genetic variation of plants was also affected by evolution in both inter- and intra-species. In addition, the morphological similarity and anatomical features of thyme cultivars create a problem for the correct identification. However, the identification of the factors responsible for the chemical polymorphism registered within species is the most challenging aspect of the essential oil analysis. vulgaris, seven different chemotypes, such as thymol, carvacrol, geraniol, linalool, thujanol-4, terpinen-4-ol, and 1,8-cineole are described. The essential oil composition might vary with the cultivar type. According to the type, the major components represent 60–95% of the total essential oil. The chemical composition of essential oils might be further altered due to cross hybridization, morphogenesis, polyploidization, extraction methods, drying conditions, stages of harvesting, etc. ![]() It was reported that the genetic constitution and environmental conditions highly influenced the yield and essential oil composition of various plant species. The essential oil components were commonly used to examine variations between populations. Therefore, it is important to validate the methods for the identification of the Korean native thyme cultivar, T. quinquecostatus cultivar is often mixed with other thyme cultivars during cultivation and in nature. However, most thyme cultivars are not yet chemically or genetically characterized. Ĭurrently, thyme seeds are commercially available in the market. quinquecostatus essential oil are thymol, γ-terpinene, and p-cymene. Owing to its medicinal and aromatic properties, it is also broadly used in pharmaceutical and food industries. quinquecostatus is extensively used in cosmetic industries for fragrance purposes. quinquecostatus has antioxidant, antimicrobial, insecticidal, immunological, antidiabetic, and antitumor properties. Recent scientific studies reported that T. quinquecostatus is used for the treatment of cough, inflammation, preventing excessive intestinal gas, and diaphoresis. Among these, Thymus quinquecostatus (Bak-ri-hyang) is a scrubby subshrub and an important aromatic plant in Korea. They are widely used as spices, herbal tea, and insecticide in addition to flavor and fragrance materials. The genus Thymus (Lamiaceae) consists of approximately 300 species of herbaceous perennials and sub-shrubs, distributed throughout the world and predominantly found in the Mediterranean basin. quinquecostatus from other Thymus species. The findings of the present study suggest that RAPD analysis can be a useful tool for marker-assisted identification of T. Among three Korean cultivars, the Wolchul and Odae cultivars were placed into the same cluster, but they did not show identical clustering with their essential oil compositions. According to the RAPD patterns, the nine thyme cultivars could be divided into two major clusters. The highest dissimilarity and similarity coefficient of the RAPD markers were 0.58 and 0.98, respectively. Cluster analysis of RAPD markers established the presence of clear separation from nine thyme cultivars. In total, 133 bands were obtained using 16 primers, and 124 bands were polymorphic, corresponding to 93.2% polymorphism. The RAPD analysis demonstrated that all thyme cultivars showed characteristic RAPD patterns that allowed their identification. ![]() Further, linalool was the most abundant component in carpet and Jiri cultivars. Whereas the Odae, lemon, and silver cultivars belong to the thymol chemotype. The GC–MS analysis revealed that Wolchul, creeping, golden, and orange cultivars belong to the geraniol chemotype. The random amplified polymorphic DNA (RAPD) analysis was accomplished using 16 different primers. The composition of essential oils obtained from nine individuals was analyzed by gas chromatography–mass spectrometry (GC–MS). ![]() vulgaris), to distinguish Bak-ri-hyang from other thyme cultivars. quinquecostatus cultivars collected from the Wolchul, Jiri, and Odae mountains, in comparison with six commercial thyme cultivars ( T. Hence, this study aimed to determine the genetic variability and the essential oil composition of three Korean native thyme, T. quinquecostatus population and is always mixed with other thyme cultivars during cultivation and marketing. Bak-ri-hyang ( Thymus quinquecostatus Celak.) is an important medicinal and aromatic plant in Korea. ![]()
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